Impact of Zinc and Selenium on Reproduction Profile of Hybrid Red Tilapia Treated with Sub-lethal Concentration of Water-Soluble Fractions (WSFs) of Petroleum Crude Oil

Document Type : Original Article

Authors

1 Faculty of Fish Resources, Suez University

2 National Institute of Oceanography and Fisheries

3 Suez Canal University, Faculty of Agriculture

4 Suez University, Faculty of Fish Resources

5 Suez Canal University, Faculty of Aveterinary

Abstract

After determination of LC50, sublethal concentration of water soluble fractions (WSFs) of petroleum crude oil -quarter of LC50- and same concentration of zinc and selenium ‘1.3 mg/l was used to study their effects on reproduction profile of hybrid red tilapia. The experiment was continued for 2 months and the groups were “control, WSFs of petroleum crude oil, zinc, selenium, zinc+ WSFs of petroleum crude oil and selenium+ WSFs of petroleum crude oil”. Triplicates of each treatment were established and fish was fed twice/daily on commercial diet 30% protein. By experiment’s end, sex ratio was calculated, gonads were obtained for Gonado-somatic index ’GSI’, biochemical and histological analysis. Sex ratio was around the normal, 3 females :1 male; results of female GSI showed no significant different between treatments while male GSI showed significant different between treatments compared to control. Steroid’s hormones ‘estradiol, testosterone and progesterone’ showed significant differences between treatments compared to control which has no significant differences with other treatments. The histological analysis showed changes in reproduction organs (testes and ovary) tissues in WSFs of petroleum crude oil treatments, while these changes were eliminated in treatments with WSFs of petroleum crude oil treatment + zinc or selenium; in zinc and selenium treatments the tissue structure closed to be normal compared to the control group.

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